2 edition of Molecular analysis of a lipoprotein, TpN24-28, from Treponema pallidum subspecies found in the catalog.
Molecular analysis of a lipoprotein, TpN24-28, from Treponema pallidum subspecies
Lesley J. Tagg
Thesis (Ph.D) - University of Birmingham, School of Biological Sciences.
|Statement||by Lesley J. Tagg.|
Apolipoprotein E, a main apoprotein of the chylomicron, binds to a specific receptor on liver cells and peripheral cells. ApoE is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. The APOE gene is mapped to chromosome 19 in a cluster with APOC1 and APOC2. Abstract. Treponema pallidum, the bacterial agent of syphilis, cannot be cultivated in constraint has severely impeded the study of the membrane biology of this complex human pathogen. A structure-to-function approach thus was adopted as a means of discerning the likely function of Tp, a kDa cytoplasmic membrane-associated lipoprotein of T. pallidum formerly designated as TmpC.
advanced search; structure search; cert of analysis; sds search; sigma-aldrich ®. SUMMARY Syphilis is a chronic disease with a waxing and waning course, the manifestations of which have been described for centuries. It occurs worldwide, and the incidence varies significantly with geographic location. Transmission is mainly by sexual contact. The causative organism, Treponema pallidum, was first described in , but because of the inability to culture the organism and the.
Low-density lipoprotein (LDL), or “bad cholesterol,” is typically associated with an increased risk of heart attack and stroke. Lipoproteins are substances made up of protein and fat. Exosporium A lipoprotein membrane containing some carbohydrate. Variations in endospore morphology: (1, 4) central endospore; (2, 3, 5) terminal endospore; (6) lateral endospore Endospore The appearance of the mature spores varies according to the species, being spherical, ovoid or elongated, occupying a terminal, subterminal or central.
Utility of natural history
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Inat the American Centers for Disease Control and Prevention (CDC), Pillay and colleagues developed the first molecular method to be ever applied to group T. pallidum strains into discrete typing units .Their method relied on the identification of 1) the number of 60 bp-repeats in the T.
pallidum a cidic r epeat p rotein (arp) gene (tp) by means of amplification of the repeat Author: Mark C. Fernandez, Lorenzo Giacani. Subsequent molecular analysis revealed that one individual, SP (unborn, 29 amen-orrhea weeks, inhumation date –), exhibited positive signals for the five T.
pallidum amplification. Strugnell R, Cockayne A, Penn CW. Molecular and antigenic analysis of treponemes. Crit Rev Microbiol. ; 17 (4)– Swancutt MA, Radolf JD, Norgard MV. The kilodalton membrane immunogen of Treponema pallidum is a lipoprotein. Infect Immun. Feb; 58 Cited by: The 16SS intergenic spacers of Treponema pallidum subspecies pallidum, Nichols strain, and Treponema pallidum subspecies pertenue, Gauthier strain.
Cutaneous immunobiology and spirochetal molecular biology have allowed investigators to propose a conceptual framework for the development of both the innate and adaptive immune response to Treponema pallidum infection.
While some clinical manifestations can be attributed to humoral responses, most can be attributed to a combination of local innate and adaptive cellular Cited by: 85 Treponema pallidum Lola V. Stamm University of North Carolina at Chapel Hill, North Carolina, USA Classification Identification and Detection Structure Metabolism and Physiology Pathogenesis Treponema pallidum subsp, pallidum is the causative agent of syphilis, a sexually transmitted infection (STI) with diverse clinical manifestations that occur in distinct.
Abstract. The Tp34 (TP) membrane lipoprotein of Treponema pallidum, an obligate human pathogen and the agent of syphilis, was previously reported to have lactoferrin binding the non-cultivatable nature of T.
pallidum, a structure-to-function approach was pursued to clarify further potential relationships between the Tp34 structural and biochemical properties and its. Abstract.
The spirochaete Treponema pallidum subsp. pallidum, the causative agent of the venereal disease syphilis, has been the subject of extensive studies ever since its discovery over eighty years study of this pathogen has been hampered by inability to cultivate the pathogenic treponemes in last decade a number of investigators have used modern molecular biology.
Syphilis, caused by the spirochete Treponema pallidum, is a chronic sexually transmitted disease which infects 12 million people ema pallidum is highly invasive and undergoes widespread dissemination via the circulatory system.
Similar to other invasive pathogens, T. pallidum has been shown to express a host-component-degrading protease, pallilysin, that binds and degrades human. mature lipoprotein of 17 kDa of the spirochete Treponema pallidum. PRESENTATION: liquid protein solution SOURCE: Escherichia coli MOLECULAR WEIGHT: determined by SDS-PAGE, the protein band is between molecular markers of 45, Da, while relative molecular mass calculated from amino acid sequence is Da.
Seminar Vol March 2, Yaws Oriol Mitjà, Kingsley Asiedu, David Mabey Yaws is an infectious disease caused by Treponema pallidum pertenue—a bacterium that closely resembles the causative agent of syphilis—and is.
1. Introduction. The genus Treponema (Krieg et al., ) comprises several human pathogens that cause chronic infections including several species of oral treponemes (e.g. denticola, T. parvum, and T.
putidum;Visser and Ellen, ).Another member of the genus Treponema, Treponema pallidum subspecies pallidum (TPA) is the causative agent of the sexually transmitted disease. BACKGROUND: Although syphilis rates have been relatively high in Italy for more than 15 years, no data on the molecular types of Treponema pallidum subspecies pallidum circulating in this country.
LIPA: Lipoprotein (a) (Lp[a]) consists of an LDL particle that is covalently bound to an additional protein, apolipoprotein (a) (Apo[a]). Apo(a) has high-sequence homology with the coagulation factor plasminogen and, like LDL, Lp(a) contains apolipoprotein B (ApoB).
Thus, Lp(a) is both proatherogenic and prothrombotic. Lp(a) is an independent risk factor for coronary heart disease (CHD. kDa LIPOPROTEIN OF T. PALLIDUM gation, washedtwice in M9labeling medium,and suspended to the original volume in labeling medium.
The cells were starved ofmethionine by incubation at 37°C for 45 min and were pulse-labeled with Tran35S-label (40 pCi/ml) for 1 min. Thechasewasinitiated bythe additionofa volumeof4% unlabeled methionine. Samples ( ml). Treponema pallidum (strain Nichols) Status.
Reviewed-Annotation score: Experimental evidence at The outer membrane of um has no lipopolysaccharides, few proteins and is a fluid and very fragile bilayer.
Its lack of surface antigenicity is thought to contribute to the ability of the pathogen to evade the human immune system. Lipoprotein analysis is also essential in monitoring the effectiveness of cholesterol‐lowering therapy. The focus of this chapter will be on methods for lipoprotein analysis that are used for routine diagnostic purposes, but in addition, new promising types of lipoprotein analysis that are still in the realm of basic research will also be.
This chapter reports on three pathogenic spirochetes: Treponema pallidum, causative agent of syphilis, and other treponemes; Borrelia burgdorferi, causative agent of Lyme disease, and related species; and Leptospira species, responsible for leptospirosis.
The spirochetes are quite different from many of the other well-studied pathogens that are principally found in the proteobacteria and gram. Yaws is an infectious disease caused by Treponema pallidum pertenue—a bacterium that closely resembles the causative agent of syphilis—and is spread by skin-to-skin contact in humid tropical regions.
Yaws causes disfiguring, and sometimes painful lesions of the skin and bones. As with syphilis, clinical manifestations can be divided into three stages; however, unlike syphilis, mother-to. Therefore, genetic analysis could be useful in the differential diagnosis of Treponema species and subspecies.
Complete genomes of T. subsp. pallidum and T. p subsp. pertenue, which cannot be distinguished morphologically, show % identity [ 12 ]. There are no reviews for Lipoprotein Lipase/LPL Recombinant Protein Antigen (NBPPEP).
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Other closely related treponemes cause pinta (Treponema carateum), yaws (Treponema pallidum subspecies pertenue), and bejel (Treponema pallidum subspecies endemicum). In o cases of primary and secondary syphilis in the United States were reported to the U.S. Centers for Disease Control and Prevention.Mass (g) = Concentration (mol/L) x Volume (L) x Molecular Weight (g/mol) As an example, if the molecular weight of a compound is g/mol and the desired concentration is 10 mM for 10 ml of water based stock solution, the required mass would be = (value determined by this calculator).